Proteomic evidence for the silk fibroin genes of spider mites (Order Trombidiformes: Family Tetranychidae)

Spider mites are a group of arachnids belonging to Acari (mites and ticks), family Tetranychidae, known produce nanoscale silk fibers characterized by high Young's modulus. The fibroin gene spider has been computationally predicted through genomic analysis Tetranychus urticae Koch, but it yet be confirmed proteomic evidence. In this work, we sequenced assembled the transcriptome from two genera mites, kanzawai Kishida Panonychus citri (McGregor), combined with proteomics T. P. characterize genes comparative genomics multiomics analysis. As result, fibroins were identified, which different than those previously computational methods. amino acid composition secondary structure suggest similarity aciniform or cylindrical spidroins silk, partly mirrors their mechanical properties, exhibiting availability full-length sequences facilitates study evolution that sometimes emerge in multiple lineages convergent manner industrial application artificial protein sequence resulting properties these silks. Here citri, mite is especially its extremely fine nano-scale diameter modulus, even exceeding genes, independently evolved insects, spiders show convergence,